Journal: Med (New York, N.y.)

Article Title: Omicron neutralizing antibody response following booster vaccination compared with breakthrough infection

doi: 10.1016/j.medj.2022.09.001

Figure Lengend Snippet:

Article Snippet: EIA/RIA high binding ELISA plates , Corning , Ref #359096.

Techniques: Virus, Recombinant, Software, Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Cancers

Article Title: T Cells Expressing a TCR-Like Antibody Selected Against the Heteroclitic Variant of a Shared MAGE-A Epitope Do Not Recognise the Cognate Epitope

doi: 10.3390/cancers12051255

Figure Lengend Snippet: Characterisation of phage-antibody clones for binding to mMA peptides. ( A ) Streptavidin plates were coated with biotinylated soluble mMA-GV/A2. Eighteen phage-antibodies were selected and incubated with titrated amounts of pMHC complexes, and binding was detected per ELISA. A representative example is shown out of two independent experiments. ( B ) Six binders from A were tested for binding towards BSM cells pulsed with mMA-GV or irrelevant peptide by flow cytometry. Data are presented as histograms and percentages in plots indicate binding towards BSM cells loaded with mMA-GV peptide corrected for irrelevant peptides.

Article Snippet: Streptavidin pre-coated 96-well ELISA plates (SanBio, Uden, The Netherlands) were coated overnight (4 °C) or 1 h at RT with 0.1 μg/mL biotinylated pMHC in PBS.

Techniques: Clone Assay, Binding Assay, Incubation, Enzyme-linked Immunosorbent Assay, Flow Cytometry